Ana Gene Biotech
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- Detection of BRAF V600E mutation,
- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Real-time PCR ARMS TaqMan Probe Technology,
- Highly Specific and Sensitive Detection Kit.

Catalog Number Description
- K2036: 2X version including 24 ready to use tubes contain 10 μl of qPCR mix 2X (two sets)
- K2036L: Lyophilized version including 48 ready to use lyophilized qPCR mix (two sets)

Lyophilized Version Price :
Old Price :
    •  Jan 2017
    •  2017


BRAF is a serine/threonine kinase that functions within the Ras-Raf-MEK-MAPK pathway. This pathway
normally regulates cell proliferation and survival under the control of growth factors and hormones.
Mutations in the BRAF gene have been associated with the development of cancer. The most common
alteration in the BRAF gene is a mutation called V600E, which alters the valine at position 600 in the
protein to a glutamic acid. The V600E mutation causes the BRAF protein to be permanently activated,
even in the absence of growth factors.

BRAF MutID Kit (Qualitative Mutation Analysis Real-time PCR Kit) is an in-vitro nucleic acid amplification
test for the qualitative detection of V600E mutations in human clinical samples. The kit contains all the
reagents necessary for qualitative mutations analysis. The assay is based on Real time PCR with
Taqman probe chemistry. The mutation and Internal Control (ABL gene) are detected in the FAM and
YELLOW channel respectively.


- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Positive Control
- Water Nuclease free
- Quick Protocol
- mini CD


I. Sensitivity
These reagents detect 10 copies of BRAF mutations. Sensitivity of the assay was determined by serially
diluting the positive control of DNA from 100 to 1 copy/PCR. Sensitivity of the assay was determined as 10
copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be detected.
II. Module Specificity
Test result will be positive, only to BRAF mutation. The primers and probes were checked for possible
homologies to all published sequences (Genbank) by BLAST analysis to avoid any homology with other
organisms. In order to check the specificity of this kit different normal/healthy DNA were analyzed. None of
these samples led to a positive band in 1.5% (w/v) agarose gel electrophoresis.
III. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products. Thus, users
can get reproducible data even though they use different batch of product.
IV. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer using
hematopoietic cell lines, blood positive and negative samples.
V. Speed
Total reaction time is less than 2 hour.


This Kit is intended for molecular biology applications and is not intended for the prevention or treatment of a disease.
All reagents may exclusively be used for in vitro diagnostics.
The product is to be used by personnel specially instructed and trained in for the in-vitro diagnostics procedures only.
It is important to pipette the indicated quantities, and mix well after each reagent addition. Check pipettes regularly.
Instructions must be followed correctly in order to obtain correct results.
This test has been validated for use with the reagents provided in the kit. The use of other Reagents or methods, or the use of equipment not fulfilling the specifications, may render equivocal results.
Detection of mutation depends on the number of fusion transcripts present in the sample, and can be affected by sample collection methods, patient-related factors (e.g. age, symptoms) or for infection stage and sample size.
Cross contamination between samples and exogenous DNA can only be avoided by following good laboratory practice.
Attention should be paid to expiration dates printed on the kit box. Do not use expired product.


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  • Product Code: 100
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