Ana Gene Biotech
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- Detection of 8 mutation of NRAS Codon 12 and 13
- Real-time PCR ARMS TaqMan Probe Technology,
- Highly Specific and Sensitive Detection Kit.

Catalog Number Description (12 reactions)
- K2037: 2X version , 12 samples
- K2037L: Lyophilized version , 12 samples (containing Lyophilized qPCR Mixes)
Lyophilized Version Price :
Old Price :
    •  Jan 2017
    •  2017


Mutations in NRAS account for about 15% of all RAS mutations in human tumors. The presence of NRAS mutations correlates with a lack of response to certain EGFR inhibitor cancer therapies such as cetuximab and panitumimab. Currently, the only KRAS FDA-approved test for use with cetuximab and panitumumab is “Amplified Refractory Mutation System Polymerase Chain Reaction” (ARMS PCR) method, with reported sensitivity of approximately 1% to 5%. These mutations appear early in carcinogenesis, mostly between the stages of early and intermediate adenoma, maintaining a constant incidence in late adenomas and in carcinomas. Using ARMS technologies, the NRAS MutID Kit enables detection of 8 mutations of NRAS codon 12 and 13 in a background of wild-type genomic DNA including:

Codon12 qPCR Mix: G12C, G12V, G12D, G12S, G12A

Codon13 qPCR Mix: G13R, G13D, G13V


- Codon 12 qPCR Mix
- Codon 13 qPCR Mix
- Normal qPCR Mix
- Dilution Buffer (only for lyophilized version)
- Positive Control
- Water Nuclease free
- Quick Protocol
- mini CD


I. Sensitivity
These reagents detect 10 copies of NRAS mutations. Sensitivity of the assay was determined by serially
diluting the positive control of DNA from 100 to 1 copy/PCR. Sensitivity of the assay was determined as 10
copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be detected.
II. Module Specificity
Test result will be positive, only to NRAS mutation. The primers and probes were checked for possible
homologies to all published sequences (Genbank) by BLAST analysis to avoid any homology with other
organisms. In order to check the specificity of this kit different normal/healthy DNA were analyzed. None of these samples led to a positive band in 1.5% (w/v) agarose gel electrophoresis.
III. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products. Thus, users
can get reproducible data even though they use different batch of product.
IV. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer using
hematopoietic cell lines, blood positive and negative samples.
V. Speed
Total reaction time is less than 2 hour.


This Kit is intended for molecular biology applications and is not intended for the prevention or
treatment of a disease.
All reagents may exclusively be used for in vitro diagnostics.
The product is to be used by personnel specially instructed and trained in for the in-vitro
diagnostics procedures only.
It is important to pipette the indicated quantities, and mix well after each reagent addition. Check
pipettes regularly.
Instructions must be followed correctly in order to obtain correct results.
This test has been validated for use with the reagents provided in the kit. The use of other Reagents
or methods, or the use of equipment not fulfilling the specifications, may render equivocal results.
Detection of mutation depends on the number of fusion transcripts present in the sample, and can be
affected by sample collection methods, patient-related factors (e.g. age, symptoms) or for infection
stage and sample size.
Cross contamination between samples and exogenous DNA can only be avoided by following good
laboratory practice.
Attention should be paid to expiration dates printed on the kit box. Do not use expired product.


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