Ana Gene Biotech
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HBV ViruFind Kit

- Detection of Hepatitis B Virus (HBV)
- Real-time PCR TaqMan Probe Technology,
- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Highly Sensitive Detection Kit.

Catalog Number Description
- K2050: 2X version including 24 ready to use tubes contain 10 ul of qPCR mix 2X
- K2050L: Lyophilized version including 24 ready to use lyophilized qPCR mix

Lyophilized Version Price :
$72
Old Price :
$0
Amount
    •  
    •  Jan 2017
    •  
    •  2017

HBV ViruFind Kit

The Hepatitis B virus (HBV) with a 3.2kbp long and partially double stranded DNA genome is a member of the Hepadnaviridae family. It can cause acute or chronic liver disease. HBV is mainly transmitted via blood or blood products. Worldwide an estimated 2 billion people have been infected with the HBV and more than 350 million suffer chronic consequences from the disease, and approximately 1 million die annually of HBV-related disease. HBV is differentiated into many genotypes, according to genome sequence. To date, eight well-known genotypes (A-H) of the HBV genome have been defined. Moreover, two new genotypes, I and J, have also been identified. Hepatitis B virus tests may be used for a variety of reasons. Some of the tests detect antibodies produced in response to HBV infection; some detect antigens produced by the virus, and others detect viral DNA. PCR tests have been developed to detect and measure the amount of HBV DNA, called the viral load, in clinical specimens. These tests are used to assess a person infection status and to monitor treatment.
HBV ViruFind Kit (Qualitative Analysis Real-time PCR Kit) is an in-vitro nucleic acid amplification test for the qualitative detection of 8 different HBV genotypes (for genotype A~H) in human clinical samples.


HBV ViruFind Kit

- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Positive Control
- DNA-IC (DNA Internal Control)
- Water Nuclease free
- Quick Protocol
- mini CD


HBV ViruFind Kit

I. Sensitivity
These reagents detect 10 copies of HBV. Sensitivity of the assay was determined by serially
diluting the positive control of DNA from 100 to 1 copy/PCR. Sensitivity of the assay was
determined as 10 copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be
detected.
II. Module Specificity
Test result will be positive, only to HBV. The primers and probes
were checked for possible homologies to all published sequences (Genbank) by BLAST analysis to
avoid any homology with other organisms.
III. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products.
Thus, users can get reproducible data even though they use different batch of product.
IV. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer
using serum positive and negative samples.
V. Speed
Total reaction time is less than 2 hour.

HBV ViruFind Kit

This Kit is intended for molecular biology applications and is not intended for the prevention or
treatment of a disease.
All reagents may exclusively be used for in vitro diagnostics.
The product is to be used by personnel specially instructed and trained in for the in-vitro
diagnostics procedures only.
It is important to pipette the indicated quantities, and mix well after each reagent addition. Check
pipettes regularly.
Instructions must be followed correctly in order to obtain correct results.
This test has been validated for use with the reagents provided in the kit. The use of other Reagents
or methods, or the use of equipment not fulfilling the specifications, may render equivocal results.
Detection of mutation depends on the number of fusion transcripts present in the sample, and can be
affected by sample collection methods, patient-related factors (e.g. age, symptoms) or for infection
stage and sample size.
Cross contamination between samples and exogenous DNA can only be avoided by following good
laboratory practice.
Attention should be paid to expiration dates printed on the kit box. Do not use expired product.

HBV ViruFind Kit



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  • Product Code: 104
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  • Product Code: 104
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