Ana Gene Biotech
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HCV ViruFind

- Detection of Hepatitis C virus (HCV)
- Real-time PCR TaqMan Probe Technology,
- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- One step cDNA synthesis and Real-time PCR,
- Highly Sensitive Detection Kit.

Catalog Number Description
- K2051S: One step qPCR including 24 ready to use tubes contain 25ul of qPCR mix 2X without Reverse Transcriptase
- K2051SL: One step qPCR including 24 ready to use lyophilized qPCR mix (Reverse Transcriptase included)
Lyophilized Version Price :
$100
Old Price :
$0
Amount
    •  
    •  Jan 2017
    •  
    •  2017

HCV ViruFind

The Hepatitis C virus (HCV) is a positive sense, single-stranded RNA virus, with a genome of 9,500 nucleotides. It has been transmitted primarily through intravenous drug use and through blood products. Worldwide, more than one million new cases of infection are reported annually. About 75 – 85% of HCV-infected individuals develop chronic hepatitis, with up to 20% of chronically infected individuals developing cirrhosis.
Hepatitis C virus has at least six forms or genotypes. It is thought that genetic heterogeneity of HCV may account for some of the differences in disease outcome and response to treatment observed in HCV-infected persons. These genotypes and subtypes are distributed variously in different parts of the world. HCV is diagnosed through several blood tests which are available. The first test that most doctors will use in the diagnosis of chronic Hepatitis C is an ELISA. It tests for antibodies against the Hepatitis C virus. Approximately 40 percent of those people who test positive for Hepatitis C by this screening blood test actually do not have Hepatitis C. This is known as a false positive test. When people with early infections have not developed antibody levels high enough that the test can measure, a false negative occurs. In addition, some people may lack the immune response necessary for the test to work well. Among the methods most commonly used for detection, assays based on real-time PCR technology offer the great sensitivity and specificity. HCV ViruFind Kit (Qualitative Mutation Analysis Real-time PCR Kit) is an in-vitro nucleic acid amplification test for the qualitative detection of CMV in human clinical samples. The primers are designed to hybridize to the 5 utr region with the fewest possible mismatches among HCV genotypes 1, 2, 3, 4, 5, and 6.The HCV sample and Internal Control (RNA-IC) are detected in the FAM and JOE channel respectively.


HCV ViruFind

- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Positive Control
- RNA-IC (10 Lyophilized RNA Control tubes)
- Water Nuclease free
- Quick Protocol
- mini CD


HCV ViruFind

I. Sensitivity
These reagents detect 10 copies of HCV. Sensitivity of the assay was determined by
serially diluting the positive control of DNA from 100 to 1 copy/PCR. Sensitivity of the assay was
determined as 10 copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be
detected.
II. Module Specificity
Test result will be positive, only to HCV. The primers and probes were checked for
possible homologies to all published sequences (Genbank) by BLAST analysis to avoid any
homology with other organisms.
III. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products.
Thus, users can get reproducible data even though they use different batch of product.
IV. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer
using serum positive and negative samples.
V. Speed
Total reaction time is less than 2 hour.

HCV ViruFind

This Kit is intended for molecular biology applications and is not intended for the prevention or
treatment of a disease.
All reagents may exclusively be used for in vitro diagnostics.
The product is to be used by personnel specially instructed and trained in for the in-vitro
diagnostics procedures only.
It is important to pipette the indicated quantities, and mix well after each reagent addition. Check
pipettes regularly.
Instructions must be followed correctly in order to obtain correct results.
This test has been validated for use with the reagents provided in the kit. The use of other Reagents
or methods, or the use of equipment not fulfilling the specifications, may render equivocal results.
Detection of mutation depends on the number of fusion transcripts present in the sample, and can be
affected by sample collection methods, patient-related factors (e.g. age, symptoms) or for infection
stage and sample size.
Cross contamination between samples and exogenous DNA can only be avoided by following good
laboratory practice.
Attention should be paid to expiration dates printed on the kit box. Do not use expired product.

HCV ViruFind



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  • Product Code: 105
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  • Old Price: $0
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  • Ex Tax: $0.0
  • Product Code: 105
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