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INF H1N1/H3N2 ViruFind Kit

- Detection of Influenza H1N1 and H3N2 in one reaction,
- Real-time PCR TaqMan Probe Technology,
- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- One step cDNA synthesis and Real-time PCR,
- Highly Sensitive Detection
- RNA Control (RNA-IC)

Catalog Number Description
- K2056S: One step qPCR including 24 ready to use tubes contain 25 ul of qPCR mix 2X without Reverse Transcriptase
- K2056SL: One step qPCR including 24 ready to use lyophilized qPCR mix (Reverse Transcriptase included)
Lyophilized Version Price :
$128
Old Price :
$0
Amount
    •  
    •  Jan 2017
    •  
    •  2017

INF H1N1/H3N2 ViruFind Kit

Influenza viruses belong to the Orthomyxoviridae that are RNA family and are divided into types A, B and C. Influenza types A and B are responsible for epidemics of respiratory illness that are often associated with increased rates of hospitalization and death. Influenza, commonly known as the flu is highly contagious and usually spread by the coughs and sneezes of a person who is infected.
Influenza A viruses are the most virulent human pathogens among the three influenza types and cause the severest disease. The influenza A virus can be subdivided into different serotypes based on the antibody response to these viruses. Among the different influenza A viruses subtypes, three major hemagglutinin subtypes (H1, H2, and H3) and two neuraminidase subtypes (N1 and N2) have evolved to human species-specific subtypes. H1N1 caused Spanish Flu in 1918 and Swine Flu in 2009 and H3N2 caused Hong Kong Flu in 1968. H1N1 typically presents with a combination of upper and lower respiratory signs and symptoms, including fever, headache, myalgia and general malaise. Duration of illness is 2-5 days, but symptoms may last for a week or longer. H3N2 infection is more severe than H1N1 in terms of fever, leukopenia, and C-reactive protein. Myalgia and other symptoms such as fever, headache, general malaise and sore throat are equally frequent in influenza A H3N2.
A variety of serological tests including the hemagglutination inhibition (HAI) test, complement fixation, and enzyme immunoassay (EIA) were used for testing paired acute- and convalescent-phase sera for diagnosing infections. Among the methods most commonly used for detection, assays based on real-time PCR technology offer the great sensitivity and specificity. Influenza ViruFind Kit (Qualitative Analysis Real-time PCR Kit) is an in-vitro nucleic acid amplification test for the qualitative detection of influenza A in human clinical samples. The assay is based on Real time PCR with Taqman probe chemistry. The FLU detection, Internal Control (DNA-IC) and cDNA Synthesis Control (Lyophilized RNA) are detected in the FAM, JOE and Red channel respectively.


INF H1N1/H3N2 ViruFind Kit

- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Positive Control
- RNA Control Tube (10 Lyophilized RNA Control tubes)
- Water Nuclease free
- Quick Protocol
- mini CD


INF H1N1/H3N2 ViruFind Kit

I. Sensitivity
These reagents detect 10 copies of INF A/B. These reagents detect 10 copies of HCV. Sensitivity of the assay was determined by
serially diluting the positive control of RNA from 100 to 1 copy/PCR. Sensitivity of the assay was
determined as 10 copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be
detected.
II. Module Specificity
Test result will be positive, only to INF A or INF B. The primers and probes
were checked for possible homologies to all published sequences (Genbank) by BLAST analysis to
avoid any homology with other organisms.
III. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products.
Thus, users can get reproducible data even though they use different batch of product.
IV. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer
using positive and negative samples.
V. Speed
Total reaction time is less than 2 hour.

INF H1N1/H3N2 ViruFind Kit

This Kit is intended for molecular biology applications and is not intended for the prevention or
treatment of a disease.
All reagents may exclusively be used for in vitro diagnostics.
The product is to be used by personnel specially instructed and trained in for the in-vitro
diagnostics procedures only.
It is important to pipette the indicated quantities, and mix well after each reagent addition. Check
pipettes regularly.
Instructions must be followed correctly in order to obtain correct results.
This test has been validated for use with the reagents provided in the kit. The use of other Reagents
or methods, or the use of equipment not fulfilling the specifications, may render equivocal results.
Detection of mutation depends on the number of fusion transcripts present in the sample, and can be
affected by sample collection methods, patient-related factors (e.g. age, symptoms) or for infection
stage and sample size.
Cross contamination between samples and exogenous DNA can only be avoided by following good
laboratory practice.
Attention should be paid to expiration dates printed on the kit box. Do not use expired product.

INF H1N1/H3N2 ViruFind Kit



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  • Product Code: 110
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