Ana Gene Biotech
Mouse move on Image to zoom

CMV ViruFind Kit

- Detection of Cytomegalovirus (CMV)
- Real-time PCR TaqMan Probe Technology,
- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Highly Sensitive Detection Kit.

Catalog Number Description
- K2053: 2X version including 24 ready to use tubes contain 10 ul of qPCR mix 2X
- K2053L: Lyophilized version including 24 ready to use lyophilized qPCR mix


Lyophilized Version Price :
$72
Old Price :
$0
Amount
    •  
    •  Jan 2017
    •  
    •  2017

CMV ViruFind Kit

The human Cytomegalovirus (CMV) is a member of the family Herpesviridae and belongs to the subfamily betaherpesvirinae. It consists an icosahedral capsid with a linear double-stranded DNA genome of approximately 230 kbp, a surrounding integument and an outer envelope. CMV has a worldwide distribution and infects humans of all ages, with no seasonal or epidemic patterns of transmission. It is characterized by a primary infection that generally occurs in a sub-clinical fashion in early childhood, with subsequent lifelong latent infection. However, CMV infection is important to certain highrisk groups. Major areas of concern are: (1) the risk of infection to the unborn baby during pregnancy (2) the risk of infection to people who work with children (3) the risk of infection to the immunocompromised person, such as organ transplant recipients and persons infected with human immunodeficiency virus (HIV). (4) the risk of infection to newborn babies. Therefore, early diagnosis of CMV infections is important in the management of high-risk patients.
CMV ViruFind Kit is an in-vitro nucleic acid amplification test for the qualitative detection of CMV in human clinical samples. The test is based on real-time PCR technology with Taqman probe chemistry, utilizing polymerase chain reaction (PCR) for the amplification of specific target sequences. The CMV target and Internal Control (DNA-IC) are detected in the FAM (Green) and JOE (Yellow) respectively. The Internal control is designed to efficiently control DNA extraction process and inhibition during the PCR amplification.


CMV ViruFind Kit

- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Positive Control
- DNA-IC (DNA Internal Control)
- Water Nuclease free
- Quick Protocol
- mini CD


CMV ViruFind Kit

Product Specifications
I. Sensitivity
These reagents detect 10 copies/reaction. Sensitivity of the assay was determined by serially diluting the positive control of DNA from 100 to 1 copy/PCR. Sensitivity of the assay was determined as 10 copies/reaction. This means that there is 95% probability that 10 Copies/PCR will be detected.
II. Module Specificity
Test result will be positive, only to CMV. The primers and probes were checked for possible homologies to all published sequences (Genbank) by BLAST analysis to avoid any homology with other organisms. In order to check the specificity of this kit different normal/healthy DNA were analyzed. None of these samples led to a positive band in 1.5% (w/v) agarose gel electrophoresis.
III. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products. Thus, users can get reproducible data even though they use different batch of product.
IV. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer using hematopoietic cell lines, blood positive and negative samples.
V. Speed
Total reaction time is less than 2 hour.


CMV ViruFind Kit

• CMV ViruFind Kit is not intended for screening of blood and blood products for the presence of CMV DNA or confirmation of the diagnosis of infection with CMV. This kit has been validated for use with human serum or human plasma collected in EDTA anticoagulant. Test with other sample types may result in inaccurate results. All reagents may exclusively be used for in vitro diagnostics.
• The product is to be used by personnel specially instructed and trained in for the in-vitro diagnostics procedures only.
• Do not subject reagents to 3 freeze-thaw cycles. Store all reagents on ice throughout the experiment.
• It is important to pipette the indicated quantities, and mix well after each reagent addition. Check pipettes regularly.
• Instructions must be followed correctly in order to obtain correct results.
• This test has been validated for use with the reagents provided in the kit. The use of other Reagents or methods, or the use of equipment not fulfilling the specifications, may render equivocal results.
• Detection of mutation depends on the number of mutation in the sample, and can be affected by sample collection methods, patient-related factors (e.g. age, symptoms) and/or infection stage and sample size.
• Cross contamination between samples and exogenous DNA can only be avoided by following good laboratory practice.
• Attention should be paid to expiration dates printed on the kit box. Do not use expired product.br

CMV ViruFind Kit



  • Free shipping
  • Best Price
  • Two years waranty on every product
  • Available on diffrent colors
  • Product Code: 122
  • Reward Points:
  • Availability: False
  • Old Price: $0
  • Ex Tax: $0.0
  • Ex Tax: $0.0
  • Product Code: 122
  • Reward Points:

Write a Review

Leave a comment

 

Comments (0)

Popular Brands See Our Popular Brands