Ana Gene Biotech
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BCR1 TransFind Kit

- Detection of PML/RARa, BCR1 breakpoint,
- Real-time PCR TaqMan Probe Technology,
- One step cDNA synthesis and Real-time PCR,
- 24 Lyophilized Ready to use BCR1 tubes,
- Highly Sensitive Detection Kit.

Catalog Number Description (24 reactions)
- K2043: 2X version including 24 ready to use tubes contain 10 μl of qPCR mix 2X
- K2043L: Lyophilized version including 24 ready to use lyophilized qPCR mix
- K2043S: One step qPCR including 24 ready to use tubes contain 25 μl of qPCR mix 2X
- K2043SL: One step qPCR including 24 ready to use lyophilized qPCR mix (Reverese Transcriptase included)
Lyophilized Version Price :
$91
Old Price :
$0
Amount
    •  
    •  Mar 2017
    •  
    •  2017

BCR1 TransFind Kit

Acute promyelocytic leukemia (APL) is a subtype of acute myelocytic leukemia (AML), a cancer of the blood and bone marrow. It is also known as acute progranulocytic leukemia; APL; AML with t(15;17)(q24;q21), PML-RARA and variants; FAB subtype M3 and M3 variant. In APL there is an overabundance of immature blood cells called “promyelocytes” and deficiency in mature white blood cells. 90% of APL patients consist of PML-RARA and RARA-PML type accounts for ~10% of all cases. APL is associated with an exchange of DNA from chromosome 15 and chromosome 17, creating a fusion gene PML-RARA that is a transcriptional repressor. Depending on the location of breakpoints within the PML site, intron 6, exon 6 and intron 3, the respective PML-RARa transcript subtypes referred to as long (L or BCR1), variant (V or BCR2) and short (S or BCR3), may be formed. Among the methods most commonly used for detection, assays based on real-time PCR technology offer the great sensitivity and specificity.
BCR1 TransFind Kit is based on Real-time PCR – Taqman Probe technology for detection of PML/RARa BCR1 breakpoint in human clinical samples. The fusion transcripts are detected in the FAM (Green) channel and Internal control (ABL1 gene) is detected in the HEX/JOE (Yellow) channel.


BCR1 TransFind Kit

- 24 Lyophilized Ready to use BCR1 tubes,
- Positive Control
- Water Nuclease free
- Quick Protocol
- mini CD

BCR1 TransFind Kit

I. Sample Requirement
RNA from Peripheral blood: 0.5 - 1mL
RNA from Bone from marrow: 0.5 - 1mL

II. Sensitivity
These reagents detect 10 copies of PML/RARa fusion transcripts. Sensitivity of the assay was determined
by serially diluting the positive control of PML/RARa from 100 to 1 copy/PCR. Sensitivity of the assay was
determined as 10 copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be detected.
III. Module Specificity
Test result will be positive, only to PML/RARa fusion transcripts. The primers and probes were checked for
possible homologies to all published sequences (Genbank) by BLAST analysis to avoid any homology with
other organisms.
IV. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products. Thus, users
can get reproducible data even though they use different batch of product.
V. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer using
hematopoietic cell lines, blood positive and negative samples.
VI. Speed
Total reaction time is less than 2 hour.

BCR1 TransFind Kit

1. This Kit is intended for molecular biology applications and is not intended for the prevention or treatment of a disease.

2. All reagents may exclusively be used for in vitro diagnostics.

3. The product is to be used by personnel specially instructed and trained in for the in-vitro diagnostics procedures only.

4. It is important to pipet the indicated quantities, and mix well after each reagent addition. Check pipettes regularly.

5. Instructions must be followed correctly in order to obtain correct results.

6. This test has been validated for use with the reagents provided in the kit. The use of other Reagents or methods, or the
use of equipment not fulfilling the specifications, may render equivocal results.

7. Detection of translocation depends on the number of fusion transcripts present in the sample, and can be affected by
sample collection methods, patient-related factors (e.g. age, symptoms) or for infection stage and sample size.

8. Cross contamination between samples and exogenous cDNA can only be avoided by following good laboratory
practice.

9. Attention should be paid to expiration dates printed on the kit box. Do not use expired product.

BCR1 TransFind Kit

1. Breccia M, et al. Clinical and biological features of acute promyelocytic leukemia patients developing retinoic acid syndrome during induction treatment with all-trans retinoic acid and idarubicin. 2008. Haematologica; 93 (12): 1918-20.

2. Van Dongen JJM, et al. Standardized RT-PCR analysis of fusion gene transcripts from chromosome aberrations in acute leukemia for detection of minimal residual disease. 1999. Leukemia; 13: 1901-1928.

3. McCraw B, et al. Diagnosing disseminated intravascular coagulopathy in acute promyelocytic leukemia. 2008. Clinical Journal of Oncology Nursing

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