Ana Gene Biotech
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ALL TransFind Kit

- Detection of the most important ALL translocations
- E2A/PBX1, TEL/AML1, mBCR/ABL (p190), MLL/AF4 and SIL/TAL1
- Real-time PCR TaqMan Probe Technology,
- Highly Sensitive Detection Kit.

Catalog Number Description (12 samples)
- K2001: 2X version , 12 samples
- K2001L: Lyophilized version , 12 samples (containing Lyophilized qPCR Mixes)

Lyophilized Version Price :
$247
Old Price :
$0
Amount
    •  
    •  Jan 2017
    •  
    •  2017

ALL TransFind Kit

Acute lymphoblastic leukemia (ALL) is a form of leukemia, or cancer of the white blood cells characterized by excess lymphoblasts. Malignant, immature white blood cells continuously multiply and are overproduced in the bone marrow. ALL causes damage and death by crowding out normal cells in the bone marrow, and by spreading (infiltrating) to other organs. ALL is most common in childhood with a peak incidence at 2-5 years of age, and another peak in old age. Initial symptoms are not specific to ALL, but worsen to the point that medical help is sought. They result from the lack of normal and healthy blood cells because they are crowded out by malignant and immature leukocytes (white blood cells). Different mutations have been associated with shorter or longer survival. Damage to DNA can be caused through the formation of fusion genes, as well as the dysregulation of a proto-oncogene via juxtaposition of it to the promoter of another gene, e.g. the T-cell receptor gene. It is known that some of the Cytogenetic translocations associated with specific molecular genetic abnormalities in ALL. This kit is based on Real-time PCR - Taqman Probe technology for detection of important translocations of ALL. Translocations that available in this kit including E2A/PBX1, TEL/AML1, mBCR/ABL-p190, MLL/AF4 and SIL/TAL1. The fusion transcripts are detected in the FAM channel and Internal control (ABL1 gene) is detected in the Yellow channel.

ALL TransFind Kit

- SIL/TAL1 Mix (Lyophilized or 2X)
- TEL/AML1 qPCR Mix (Lyophilized or 2X)
- E2A/PBX qPCR Mix (Lyophilized or 2X)
- BCR/ABL (P190) qPCR Mix (Lyophilized or 2X)
- MLL/AF4 qPCR Mix (Lyophilized or 2X)
- Dilution Buffer (only for lyophilized version)
- Positive Conrol Mix
- Water Nuclease free
- Quick Protocol
- mini CD

ALL TransFind Kit

I. Sample Requirement
RNA from Peripheral blood: 0.5 - 1mL
RNA from Bone from marrow: 0.5 - 1mL

II. Sensitivity
These reagents detect 10 copies of ALL associated fusion transcripts. Sensitivity of the assay was
determined by serially diluting the positive control of ALL associated fusion transcripts from 100 to 1
copy/PCR. Sensitivity of the assay was determined as 10 copies/PCR. This means that there is 95%
probability that 10 Copies/PCR will be detected.
III. Module Specificity
Test result will be positive, only to ALL associated fusion transcripts. The primers and probes were checked
for possible homologies to all published sequences (Genbank) by BLAST analysis to avoid any homology
with other organisms.
IV. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products. Thus, users
can get reproducible data even though they use different batch of product.
V. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer using
hematopoietic cell lines, blood positive and negative samples.
VI. Speed
Total reaction time is less than 2 hour.

ALL TransFind Kit

1. This Kit is intended for molecular biology applications and is not intended for the prevention or treatment of a disease.

2. All reagents may exclusively be used for in vitro diagnostics.

3. The product is to be used by personnel specially instructed and trained in for the in-vitro diagnostics procedures only.

4. It is important to pipet the indicated quantities, and mix well after each reagent addition. Check pipettes regularly.

5. Instructions must be followed correctly in order to obtain correct results.

6. This test has been validated for use with the reagents provided in the kit. The use of other Reagents or methods, or the
use of equipment not fulfilling the specifications, may render equivocal results.

7. Detection of translocation depends on the number of fusion transcripts present in the sample, and can be affected by
sample collection methods, patient-related factors (e.g. age, symptoms) or for infection stage and sample size.

8. Cross contamination between samples and exogenous cDNA can only be avoided by following good laboratory
practice.

9. Attention should be paid to expiration dates printed on the kit box. Do not use expired product.

ALL TransFind Kit

1. Leukemia--Acute Lymphocytic. American Cancer Society. Retrieved 8 February 2013

2. Caslini C, Serna A, Rossi V, Introna M, Biondi A (June 2004). Modulation of cell cycle by graded expression of MLL-AF4 fusion
oncoprotein. Leukemia 18(6): 1064-71. doi:10.1038/sj.leu.2403321. PMID 14990976.

3. Hoffbrand, Victor; Moss, Paul; Pettit, John (31 October 2006). Essential Haematology. Wiley. ISBN 978-1-4051-3649-5. Retrieved 14 September 2013.

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