Ana Gene Biotech
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CML TransFind Kit

- Detection of BCR/ABL (P210 and P230) Translocation,
- Real-time PCR TaqMan Probe Technology,
- One step cDNA synthesis and Real-time PCR,
- 24 Lyophilized Ready to use P210 tubes,
- 24 Lyophilized Ready to use P230 tubes,
- Highly Sensitive Detection Kit.

Catalog Number Description (24 reactions)
- K2003: 2X version including 24 ready to use tubes contain 10 μl of qPCR mix 2X
- K2003L: Lyophilized version including 24 ready to use lyophilized qPCR mix
- K2003S: One step qPCR including 24 ready to use tubes contain 25 μl of qPCR mix 2X
- K2003SL: One step qPCR including 24 ready to use lyophilized qPCR mix (Reverese Transcriptase included)
Lyophilized Version Price :
$175
Old Price :
$0
Amount
    •  
    •  Mar 2017
    •  
    •  2017

CML TransFind Kit

Chronic myeloid leukemia (CML) belongs to the group of Myeloproliferative Neoplasms and is in 90% of cases characterized by the presence of the Philadelphia chromosome (Ph CHRS). This chromosome is the product of a reciprocal translocation between the long arms of chromosomes 9 and 22, t(9;22), BCR (Breakpoint Cluster Region) being located on Chromosome 22 and the c-ABL oncogene coming from Chromosome 9. The different fusion proteins encoded by BCR-ABL vary in size depending on the breakpoint in the BCR gene but share a high tyrosine kinase activity, in part responsible for the leukemogenesis. Three breakpoint cluster regions in the BCR gene have been described to date: major (M-bcr or P210), minor (m-bcr or P190) and micro (u-bcr or P230).
CML TransFind Kit is based on Real-time PCR – Taqman Probe technology for detection of BCR/ABL fusions, P210 (b3a2, b2a2, b2a3 and b3a3) and P230 (e19a2 and e19a3) in human clinical samples. The fusion transcripts are detected in the FAM (Green) channel and Internal control (ABL1 gene) is detected in the HEX/JOE (Yellow) channel.


CML TransFind Kit

- 24 Lyophilized Ready to use P210 tubes (M-bcr ),
- 24 Lyophilized Ready to use P230 tubes (u-bcr),
- Positive Control
- Water Nuclease free
- Quick Protocol
- mini CD
p190 qPCR Mix (2X) (m-bcr)
p210 qPCR Mix (2X) (M-bcr )
p230 qPCR Mix (2X) (bcr)
Water Nuclease free
Quick Protocol
mini CD

CML TransFind Kit

I. Sample Requirement
RNA from Peripheral blood: 0.5 - 1mL
RNA from Bone from marrow: 0.5 - 1mL

II. Sensitivity
These reagents detect 10 copies of BCR/ABL fusion transcripts. Sensitivity of the assay was determined by
serially diluting the positive control of BCR/ABL from 100 to 1 copy/PCR. Sensitivity of the assay was
determined as 10 copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be detected.
III. Module Specificity
Test result will be positive, only to BCR/ABL fusion transcripts. The primers and probes were checked for
possible homologies to all published sequences (Genbank) by BLAST analysis to avoid any homology with
other organisms.
IV. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products. Thus, users
can get reproducible data even though they use different batch of product.
V. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer using
hematopoietic cell lines, blood positive and negative samples.
VI. Speed
Total reaction time is less than 2 hour.

CML TransFind Kit

1. This Kit is intended for molecular biology applications and is not intended for the prevention or treatment of a disease.

2. All reagents may exclusively be used for in vitro diagnostics.

3. The product is to be used by personnel specially instructed and trained in for the in-vitro diagnostics procedures only.

4. It is important to pipet the indicated quantities, and mix well after each reagent addition. Check pipettes regularly.

5. Instructions must be followed correctly in order to obtain correct results.

6. This test has been validated for use with the reagents provided in the kit. The use of other Reagents or methods, or the
use of equipment not fulfilling the specifications, may render equivocal results.

7. Detection of translocation depends on the number of fusion transcripts present in the sample, and can be affected by
sample collection methods, patient-related factors (e.g. age, symptoms) or for infection stage and sample size.

8. Cross contamination between samples and exogenous cDNA can only be avoided by following good laboratory
practice.

9. Attention should be paid to expiration dates printed on the kit box. Do not use expired product.

CML TransFind Kit

1. Chasseriau J, et al. Characterization of the different BCR-ABL transcripts with a single multiplex RT-PCR. 2004. Molecular diagnostics; 6 (4): 343-347.

2. Yaghmaie M, et al. Frequency of BCR-ABL fusion transcripts in Iranian patients with chronic myeloid leukemia. 2008. Arch Iranian Med; 11 (3): 247-251.

3. Van Dongen JJM, et al. Standardized RT-PCR analysis of fusion gene transcripts from chromosome aberrations in acute leukemia for detection of minimal residual disease. 1999. Leukemia; 13: 1901-1928.


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