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CBFb/MYH11 TransFind Kit

- Detection of CBFb/MYH11 - inv(16) Translocation,
- Real-time PCR TaqMan Probe Technology,
- One step cDNA synthesis and Real-time PCR,
- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Highly Sensitive Detection Kit.

Catalog Number Description (24 reactions)
- K2010: 2X version including 24 ready to use tubes contain 10 μl of qPCR mix 2X
- K2010L: Lyophilized version including 24 ready to use lyophilized qPCR mix
- K2010S: One step qPCR including 24 ready to use tubes contain 25 μl of qPCR mix 2X
- K2010SL: One step qPCR including 24 ready to use lyophilized qPCR mix (Reverese Transcriptase included
Lyophilized Version Price :
$91
Old Price :
$92.1
Amount
    •  
    •  Mar 2017
    •  
    •  2017

CBFb/MYH11 TransFind Kit

The pericentric inversion of chromosome 16(p13;q22), and less frequently the t(16;16) (p13;q22) translocation, accounts for 16% of the chromosomal aberrations associated with AML. This inversion results in fusion of the core binding factor beta (CBFB) gene on 16q22 with the smooth muscle myosin heavy chain gene (MYH11) on 16p13, leading to the formation of a chimeric CBFb/MYH11 fusion protein. The N-terminal of CBFb fuses to the C-terminal of MYH11 with its multimerisation domain. The resultant chimeric protein reduces the amount of active CBF. An accumulation of CBFb-MYH11/CBFa multimers in the nucleus also occurs. Clinically, the inv(16) or t(16;16) is associated with AML with abnormal eosinophils (French-American-British classification M4E0 subtype), with abnormal eosinophils being part of the malignant clone. Patients with inv(16) or t(16;16) generally have relatively good response and long-term disease-free survival rates. Among the methods most commonly used for detection, assays based on real-time PCR technology offer the great sensitivity and specificity. This kit is based on Real-time PCR - Taqman Probe technology for detection of CBFb/MYH11 - inv(16) fusion.The fusion transcripts are detected in the FAM channel and Internal control (ABL1 gene) is detected in the Yellow channel.

CBFb/MYH11 TransFind Kit

- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Positive Control
- Water Nuclease free
- Quick Protocol
- mini CD


CBFb/MYH11 TransFind Kit

I. Sample Requirement
RNA from Peripheral blood: 0.5 - 1mL
RNA from Bone from marrow: 0.5 - 1mL

II. Sensitivity
These reagents detect 10 copies of CBFb/MYH11fusion transcripts. Sensitivity of the assay was determined
by serially diluting the positive control of CBFb/MYH11 from 100 to 1 copy/PCR. Sensitivity of the assay was
determined as 10 copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be detected.
III. Module Specificity
Test result will be positive, only to CBFb/MYH11 fusion transcripts. The primers and probes were checked
for possible homologies to all published sequences (Genbank) by BLAST analysis to avoid any homology
with other organisms.
IV. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products. Thus, users
can get reproducible data even though they use different batch of product.
V. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer using
hematopoietic cell lines, blood positive and negative samples.
VI. Speed
Total reaction time is less than 2 hour.

CBFb/MYH11 TransFind Kit

1. This Kit is intended for molecular biology applications and is not intended for the prevention or treatment of a disease.

2. All reagents may exclusively be used for in vitro diagnostics.

3. The product is to be used by personnel specially instructed and trained in for the in-vitro diagnostics procedures only.

4. It is important to pipet the indicated quantities, and mix well after each reagent addition. Check pipettes regularly.

5. Instructions must be followed correctly in order to obtain correct results.

6. This test has been validated for use with the reagents provided in the kit. The use of other Reagents or methods, or the
use of equipment not fulfilling the specifications, may render equivocal results.

7. Detection of translocation depends on the number of fusion transcripts present in the sample, and can be affected by
sample collection methods, patient-related factors (e.g. age, symptoms) or for infection stage and sample size.

8. Cross contamination between samples and exogenous cDNA can only be avoided by following good laboratory
practice.

9. Attention should be paid to expiration dates printed on the kit box. Do not use expired product.

CBFb/MYH11 TransFind Kit

1.Kundu M, Chen A, Anderson S, Kirby M, Xu L, Castilla LH, Bodine D, Liu PP. Role of Cbfb in hematopoiesis and perturbations resulting from expression of the leukemogenic fusion gene Cbfb-MYH11. Blood. 2002;100:2449-2456.

2. Castilla LH, Wijmenga C, Wang Q, Stacy T, Speck NA, Eckhaus M, Marin-Padilla M, Collins FS, Wynshaw-Boris A, Liu PP. Failure of embryonic hematopoiesis and lethal hemorrhages in mouse embryos heterozygous for a knocked-in leukemia gene CBFB-MYH11. Cell. 1996;87:687-696.


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