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TEL/AML1 TransFind Kit

- Detection of TEL/AML1 - t(12;21) Translocation,
- Real-time PCR TaqMan Probe Technology,
- One step cDNA synthesis and Real-time PCR,
- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Highly Sensitive Detection Kit.

Catalog Number Description (24 reactions)
- K2006: 2X version including 24 ready to use tubes contain 10 μl of qPCR mix 2X
- K2006L: Lyophilized version including 24 ready to use lyophilized qPCR mix
- K2006S: One step qPCR including 24 ready to use tubes contain 25 μl of qPCR mix 2X
- K2006SL: One step qPCR including 24 ready to use lyophilized qPCR mix (Reverese Transcriptase included


Lyophilized Version Price :
$91
Old Price :
$92.1
Amount
    •  
    •  Mar 2017
    •  
    •  2017

TEL/AML1 TransFind Kit

This translocation fuses the TEL (ETV6) gene on chromosome 12, a member of the ETS family of transcription factors to AML-1 (RUNX1) that encodes the AML-1 transcription factor complex on chromosome 21 which is the most frequent target of myeloid associated translocations. The role of the TEL-AML1 oncoprotein in leukemogenesis is still unclear, but data suggest that it directly alters the transcriptional activity of AML1, required for normal hematopoiesis. TEL-AML1 Fusion was reported to occur in approximately 25% of childhood ALL. The majority of positive patients range in age between 1 and 10 years at diagnosis, with a peak between 2 and 5 years. Among the methods most commonly used for detection, assays based on real-time PCR technology offer the great sensitivity and specificity. This kit is based on Real-time PCR - Taqman Probe technology for detection of TEL/AML1 - t(12;21) fusion. The fusion transcripts are detected in the FAM channel and Internal control (ABL1 gene) is detected in the Yellow channel.


TEL/AML1 TransFind Kit

- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Positive Control
- Water Nuclease free
- Quick Protocol
- mini CD


TEL/AML1 TransFind Kit

I. Sample Requirement
RNA from Peripheral blood: 0.5 - 1mL
RNA from Bone from marrow: 0.5 - 1mL

II. Sensitivity
These reagents detect 10 copies of TEL/AML1fusion transcripts. Sensitivity of the assay was determined by
serially diluting the positive control of TEL/AML1 from 100 to 1 copy/PCR. Sensitivity of the assay was
determined as 10 copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be detected.
III. Module Specificity
Test result will be positive, only to TEL/AML1 fusion transcripts. The primers and probes were checked for
possible homologies to all published sequences (Genbank) by BLAST analysis to avoid any homology with
other organisms.
IV. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products. Thus, users
can get reproducible data even though they use different batch of product.
V. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer using
hematopoietic cell lines, blood positive and negative samples.
VI. Speed
Total reaction time is less than 2 hour.

TEL/AML1 TransFind Kit

1. This Kit is intended for molecular biology applications and is not intended for the prevention or treatment of a disease.

2. All reagents may exclusively be used for in vitro diagnostics.

3. The product is to be used by personnel specially instructed and trained in for the in-vitro diagnostics procedures only.

4. It is important to pipet the indicated quantities, and mix well after each reagent addition. Check pipettes regularly.

5. Instructions must be followed correctly in order to obtain correct results.

6. This test has been validated for use with the reagents provided in the kit. The use of other Reagents or methods, or the
use of equipment not fulfilling the specifications, may render equivocal results.

7. Detection of translocation depends on the number of fusion transcripts present in the sample, and can be affected by
sample collection methods, patient-related factors (e.g. age, symptoms) or for infection stage and sample size.

8. Cross contamination between samples and exogenous cDNA can only be avoided by following good laboratory
practice.

9. Attention should be paid to expiration dates printed on the kit box. Do not use expired product.

TEL/AML1 TransFind Kit

1. Nicole L. Ramakers-vanWoerden, (August 2000)TEL/AML1 gene fusion is related to in vitro drug sensitivity for L-asparaginase in childhood acute lymphoblastic leukemia. BLOOD, 1 AUGUST 2000, Vol 96, No 3

2. Lococo F, Pisegna S, Diverio D. The AML1gene a transcription factor involved in the pathogenesis of myeloid and lymphoid leukemias. Haematologica. 1997;82:364-370

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