Ana Gene Biotech
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E2A/PBX1 TransFind Kit

- Detection of E2A/PBX - t(1;19) Translocation,
- Real-time PCR TaqMan Probe Technology,
- One step cDNA synthesis and Real-time PCR,
- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Highly Sensitive Detection Kit.

Lyophilized Version Price :
$92.1
Old Price :
$107.5
Amount
    •  
    •  Jun 2016
    •  
    •  2016

E2A/PBX1 TransFind Kit

E2A-PBX1 fusion protein (also named TCF3/PBX1) contains the transactivation domain of E2A and the DNA-binding domain of PBX1 and is generated by t(1;19)(q23;p13) translocation. t(1;19) occurs in 5% of pre-B-cell acute lymphoid leukemias (ALL) in children and adults and E2A-PBX1 has been widely characterized in ALL. E2A-PBX1 can cause transformation in several cell types in vitro and induce lymphoblastic lymphomas in transgenic mice. Target genes of E2A-PBX1 includes fibroblast growth factor (FGF)-15, WNT-16, and some novel genes. The t(1;19) is detected in about 5-6% of childhood ALL and in about 3% of adult ALL. Among the methods most commonly used for detection, assays based on real-time PCR technology offer the great sensitivity and specificity. This kit is based on Real-time PCR - Taqman Probe technology for detection of E2A/PBX - t(1;19) fusion. The fusion transcripts are detected in the FAM channel and Internal control (ABL1 gene) is detected in the Yellow channel.

E2A/PBX1 TransFind Kit

- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Positive Control
- Water Nuclease free
- Quick Protocol
- mini CD


E2A/PBX1 TransFind Kit

I. Sample Requirement
RNA from Peripheral blood: 0.5 - 1mL
RNA from Bone from marrow: 0.5 - 1mL

II. Sensitivity
These reagents detect 10 copies of E2A/PBX fusion transcripts. Sensitivity of the assay was determined by
serially diluting the positive control of E2A/PBX from 100 to 1 copy/PCR. Sensitivity of the assay was
determined as 10 copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be detected.
III. Module Specificity
Test result will be positive, only to E2A/PBX fusion transcripts. The primers and probes were checked for
possible homologies to all published sequences (Genbank) by BLAST analysis to avoid any homology with
other organisms.
IV. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products. Thus, users
can get reproducible data even though they use different batch of product.
V. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer using
hematopoietic cell lines, blood positive and negative samples.
VI. Speed
Total reaction time is less than 2 hour.

E2A/PBX1 TransFind Kit

1. This Kit is intended for molecular biology applications and is not intended for the prevention or treatment of a disease.

2. All reagents may exclusively be used for in vitro diagnostics.

3. The product is to be used by personnel specially instructed and trained in for the in-vitro diagnostics procedures only.

4. It is important to pipet the indicated quantities, and mix well after each reagent addition. Check pipettes regularly.

5. Instructions must be followed correctly in order to obtain correct results.

6. This test has been validated for use with the reagents provided in the kit. The use of other Reagents or methods, or the
use of equipment not fulfilling the specifications, may render equivocal results.

7. Detection of translocation depends on the number of fusion transcripts present in the sample, and can be affected by
sample collection methods, patient-related factors (e.g. age, symptoms) or for infection stage and sample size.

8. Cross contamination between samples and exogenous cDNA can only be avoided by following good laboratory
practice.

9. Attention should be paid to expiration dates printed on the kit box. Do not use expired product.

E2A/PBX1 TransFind Kit

1.S. Jim enez-Morales. BCR-ABL, ETV6-RUNX1and E2A-PBX1 : Prevalence of the most common acute lymphoblastic leukemia
fusion genes in Mexican patients. Leukemia Research 32 (2008) 1518-1522

2. Simon E Aspland, Heather H Bendall and Cornelis Murre. The role of E2A-PBX1 in leukemogenesis. Oncogene (2001) 20,
5708-5717.

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