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MLL/AF4 TransFind Kit

- Detection of MLL/AF4 - t(4;11) Translocation,
- Real-time PCR TaqMan Probe Technology,
- One step cDNA synthesis and Real-time PCR,
- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Highly Sensitive Detection Kit.

Catalog Number Description (24 reactions)
- K2008: 2X version including 24 ready to use tubes contain 10 μl of qPCR mix 2X
- K2008L: Lyophilized version including 24 ready to use lyophilized qPCR mix
- K2008S: One step qPCR including 24 ready to use tubes contain 25 μl of qPCR mix 2X
- K2008SL: One step qPCR including 24 ready to use lyophilized qPCR mix (Reverese Transcriptase included
Lyophilized Version Price :
Old Price :
    •  Mar 2017
    •  2017

MLL/AF4 TransFind Kit

The mixed lineage leukemia 1 protein (MLL) regulates HOX gene expression through the H3 lysine 4 (K4) methyltransferase activity of its C-terminal catalytic domain. Rearrangements of the MLL gene are associated with aggressive acute leukemias, the most common of which are balanced chromosomal translocations that fuse N-terminal sequences in frame to over 50 different fusion partner proteins. MLL fusion-transformed hematopoietic progenitor cells exhibit a persistent expression of MLL target genes that is correlated with persistent H3K4 trimethylation (mediated by MLL expressed from the nonrearranged allele) and an increase in histone H3-K79 methylation by DOT1. Infant pro-B acute lymphoblastic leukemia (ALL) harboring the fusion MLL-AF4 is characterized by a very brief latency and dismal prognosis, raising the question of how this infant cancer evolves so quickly. Among the methods most commonly used for detection, assays based on real-time PCR technology offer the great sensitivity and specificity.This kit is based on Real-time PCR - Taqman Probe technology for detection of MLL/AF4 - t(4;11) fusion. The fusion transcripts are detected in the FAM channel and Internal control (ABL1 gene) is detected in the Yellow channel.

MLL/AF4 TransFind Kit

- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Positive Control
- Water Nuclease free
- Quick Protocol
- mini CD

MLL/AF4 TransFind Kit

I. Sample Requirement
RNA from Peripheral blood

II. Sensitivity
These reagents detect 10 copies of MLL/AF4 fusion transcripts. Sensitivity of the assay was determined by
serially diluting the positive control of MLL/AF4 from 100 to 1 copy/PCR. Sensitivity of the assay was
determined as 10 copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be detected.
III. Module Specificity
Test result will be positive, only to MLL/AF4 fusion transcripts. The primers and probes were checked for
possible homologies to all published sequences (Genbank) by BLAST analysis to avoid any homology with
other organisms.
IV. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products. Thus, users
can get reproducible data even though they use different batch of product.
V. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer using
hematopoietic cell lines, blood positive and negative samples.
VI. Speed
Total reaction time is less than 2 hour.

MLL/AF4 TransFind Kit

1. This Kit is intended for molecular biology applications and is not intended for the prevention or treatment of a disease.

2. All reagents may exclusively be used for in vitro diagnostics.

3. The product is to be used by personnel specially instructed and trained in for the in-vitro diagnostics procedures only.

4. It is important to pipet the indicated quantities, and mix well after each reagent addition. Check pipettes regularly.

5. Instructions must be followed correctly in order to obtain correct results.

6. This test has been validated for use with the reagents provided in the kit. The use of other Reagents or methods, or the
use of equipment not fulfilling the specifications, may render equivocal results.

7. Detection of translocation depends on the number of fusion transcripts present in the sample, and can be affected by
sample collection methods, patient-related factors (e.g. age, symptoms) or for infection stage and sample size.

8. Cross contamination between samples and exogenous cDNA can only be avoided by following good laboratory

9. Attention should be paid to expiration dates printed on the kit box. Do not use expired product.

MLL/AF4 TransFind Kit

1.Kim-Rouille MH, MacGregor A, Wiedemann LM, Greaves MF, Navarrete C. MLL-AF4 gene fusions in normal newborns. Blood 1999;93:1107-8.

2. Fatih M. Uckun, Kim Herman-Hatten. Clinical Significance of MLL-AF4 Fusion Transcript Expression in the Absence of a Cytogenetically Detectable t(4;11)(q21;q23) Chromosomal Translocation. Blood,Vol 92, No 3 (August 1), 1998: pp 810-821

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  • Old Price: $92.1
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  • Product Code: 61
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