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MLL/AF9 TransFind Kit

- Detection of MLL/AF9 - t(9;11) Translocation,
- Real-time PCR TaqMan Probe Technology,
- One step cDNA synthesis and Real-time PCR,
- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Highly Sensitive Detection Kit.

Catalog Number Description (24 reactions)
- K2011: 2X version including 24 ready to use tubes contain 10 μl of qPCR mix 2X
- K2011L: Lyophilized version including 24 ready to use lyophilized qPCR mix
- K2011S: One step qPCR including 24 ready to use tubes contain 25 μl of qPCR mix 2X
- K2011SL: One step qPCR including 24 ready to use lyophilized qPCR mix (Reverese Transcriptase included

Lyophilized Version Price :
$91
Old Price :
$92.1
Amount
    •  
    •  Mar 2017
    •  
    •  2017

MLL/AF9 TransFind Kit

The MLL gene has been found translocated to over 50 different partner genes in acute leukemia. Certain partner genes are associated with distinct leukemia subtypes, e.g. MLL-AF4 with pro B acute lymphoblastic leukemia (ALL) and MLL-AF9, -AF6 and -AF10 with acute myeloid leukemia (AML) of M4 and M5 subtypes. MLL and AF9 wildtype proteins play essential roles in embryogenesis and hematopoiesis and are parts of protein complexes leading to transcriptional initiation (MLL) and elongation (AF9) of target genes. The fusion protein MLL-AF9 is believed to combine these properties, leading to increased activation of target genes via transcriptional initiation and elongation. The MLL/AF9 fusion gene is the most frequent MLL rearrangement in childhood AML and associated with aggressive leukemia of both the myeloid and lymphoid lineage in infants, whereas in adults, this translocation is mainly associated with acute myeloid leukemia. Furthermore, MLL/AF9 may be also found in ALL of patients younger than 1-year-old (infants). New targeted therapies are needed for this type of leukemia with poor prognosis. MLL/AF9 TransFind Kit is based on Real-time PCR - Taqman Probe technology for detection of MLL/AF9 - t(9;11) fusion. The fusion transcripts are detected in the FAM channel and Internal control (ABL1 gene) is detected in the Yellow channel.


MLL/AF9 TransFind Kit

- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Positive Control
- Water Nuclease free
- Quick Protocol
- mini CD


MLL/AF9 TransFind Kit

I. Sample Requirement
RNA from Peripheral blood

II. Sensitivity
These reagents detect 10 copies of MLL/AF4 fusion transcripts. Sensitivity of the assay was determined by
serially diluting the positive control of MLL/AF4 from 100 to 1 copy/PCR. Sensitivity of the assay was
determined as 10 copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be detected.
III. Module Specificity
Test result will be positive, only to MLL/AF4 fusion transcripts. The primers and probes were checked for
possible homologies to all published sequences (Genbank) by BLAST analysis to avoid any homology with
other organisms.
IV. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products. Thus, users
can get reproducible data even though they use different batch of product.
V. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer using
hematopoietic cell lines, blood positive and negative samples.
VI. Speed
Total reaction time is less than 2 hour.


MLL/AF9 TransFind Kit

1. This Kit is intended for molecular biology applications and is not intended for the prevention or treatment of a disease.

2. All reagents may exclusively be used for in vitro diagnostics.

3. The product is to be used by personnel specially instructed and trained in for the in-vitro diagnostics procedures only.

4. It is important to pipet the indicated quantities, and mix well after each reagent addition. Check pipettes regularly.

5. Instructions must be followed correctly in order to obtain correct results.

6. This test has been validated for use with the reagents provided in the kit. The use of other Reagents or methods, or the
use of equipment not fulfilling the specifications, may render equivocal results.

7. Detection of translocation depends on the number of fusion transcripts present in the sample, and can be affected by
sample collection methods, patient-related factors (e.g. age, symptoms) or for infection stage and sample size.

8. Cross contamination between samples and exogenous cDNA can only be avoided by following good laboratory
practice.

9. Attention should be paid to expiration dates printed on the kit box. Do not use expired product.

MLL/AF9 TransFind Kit

1.Kim-Rouille MH, MacGregor A, Wiedemann LM, Greaves MF, Navarrete C. MLL-AF4 gene fusions in normal newborns. Blood 1999;93:1107-8.

2. Fatih M. Uckun, Kim Herman-Hatten. Clinical Significance of MLL-AF4 Fusion Transcript Expression in the Absence of a Cytogenetically Detectable t(4;11)(q21;q23) Chromosomal Translocation. Blood,Vol 92, No 3 (August 1), 1998: pp 810-821


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