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SIL/TAL1 TransFind Kit

- Detection of SIL/TAL Translocation [del(1p)],
- Real-time PCR TaqMan Probe Technology,
- One step cDNA synthesis and Real-time PCR,
- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Highly Sensitive Detection Kit.

Catalog Number Description (24 reactions)
- K2005: 2X version including 24 ready to use tubes contain 10 μl of qPCR mix 2X
- K2005L: Lyophilized version including 24 ready to use lyophilized qPCR mix
- K2005S: One step qPCR including 24 ready to use tubes contain 25 μl of qPCR mix 2X
- K2005SL: One step qPCR including 24 ready to use lyophilized qPCR mix (Reverese Transcriptase included
Lyophilized Version Price :
$91
Old Price :
$92.1
Amount
    •  
    •  Mar 2017
    •  
    •  2017

SIL/TAL1 TransFind Kit

Disruption of the TAL1 gene on chromosome 1p32 [del(1p)] is a common rearrangement in the development of T-cell acute lymphocytic leukemia (T-ALL). Approximately 25% of all childhood T-ALLs bear specific TAL1 gene rearrangement. These 90 kb interstitial deletions place the coding part of the TAL1 gene under the control of the first non-coding exon of the SCL interrupting locus (SIL) gene. Aberrant V(D)J recombinase activity is thought to be responsible resulting in a gain of function mutation in T-ALL. These tumor-specific rearrangements cannot be detected cytogenetically. Among the methods most commonly used for detection, assays based on real-time PCR technology offer the great sensitivity and specificity. This kit is based on Real-time PCR - Taqman Probe technology for detection of SIL/TAL fusion.The fusion transcripts are detected in the FAM channel and Internal control (ABL1 gene) is detected in the yellow channel.

SIL/TAL1 TransFind Kit

- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Positive Control
- Water Nuclease free
- Quick Protocol
- mini CD


SIL/TAL1 TransFind Kit

I. Sample Requirement
RNA from Peripheral blood: 0.5 - 1mL
RNA from Bone from marrow: 0.5 - 1mL

II. Sensitivity
These reagents detect 10 copies of SIL/TALfusion transcripts. Sensitivity of the assay was determined by
serially diluting the positive control of SIL/TAL from 100 to 1 copy/PCR. Sensitivity of the assay was
determined as 10 copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be detected.
III. Module Specificity
Test result will be positive, only to SIL/TAL fusion transcripts. The primers and probes were checked for
possible homologies to all published sequences (Genbank) by BLAST analysis to avoid any homology with
other organisms.
IV. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products. Thus, users
can get reproducible data even though they use different batch of product.
V. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer using
hematopoietic cell lines, blood positive and negative samples.
VI. Speed
Total reaction time is less than 2 hour.

SIL/TAL1 TransFind Kit

1. This Kit is intended for molecular biology applications and is not intended for the prevention or treatment of a disease.

2. All reagents may exclusively be used for in vitro diagnostics.

3. The product is to be used by personnel specially instructed and trained in for the in-vitro diagnostics procedures only.

4. It is important to pipet the indicated quantities, and mix well after each reagent addition. Check pipettes regularly.

5. Instructions must be followed correctly in order to obtain correct results.

6. This test has been validated for use with the reagents provided in the kit. The use of other Reagents or methods, or the
use of equipment not fulfilling the specifications, may render equivocal results.

7. Detection of translocation depends on the number of fusion transcripts present in the sample, and can be affected by
sample collection methods, patient-related factors (e.g. age, symptoms) or for infection stage and sample size.

8. Cross contamination between samples and exogenous cDNA can only be avoided by following good laboratory
practice.

9. Attention should be paid to expiration dates printed on the kit box. Do not use expired product.

SIL/TAL1 TransFind Kit

1.Chen X, Pan Q, Stow P, Behm FG, Goorha R, Pui CH, et al. Quantification of minimal residual disease in T-lineage acute lymphoblastic leukemia with the TAL1 deletion using a standardized real-time PCR assay. Leukemia 2001;15:166-70.

2. Delabesse E, Bernard M, Landman-Parker J, Davi F, Leboeuf D,Varet B, et al. Simultaneous SIL-TAL1 RT-PCR detection of all tal (d) deletions and identification of novel tal(d) variants. Br J Haematol 1997;99:901-7.


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  • Old Price: $92.1
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  • Product Code: 63
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