Ana Gene Biotech
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JAK2 MutID

- Detection of JAK2 V617F mutation,
- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Real-time PCR ARMS TaqMan Probe Technology,
- Highly Specific and Sensitive Detection Kit.

Catalog Number Description
- K2012: 2X version including 24 ready to use tubes contain 10 μl of qPCR mix 2X (two sets)
- K2012L: Lyophilized version including 24 ready to use lyophilized qPCR mix (two sets)
Lyophilized Version Price :
$100
Old Price :
$0
Amount
    •  
    •  Jan 2017
    •  
    •  2017

JAK2 MutID

Description
An acquired single nucleotide mutation affecting the Janus Tyrosine Kinase 2 or JAK2 (V617F- c.1849GT) was first identified in 2005 in patients suffering from polycythemia vera. JAK2 V617F is a gain-of-function mutation that leads to clonal proliferation. Occuring in the pseudokinase domain of JAK2 in hematopoietic cells, this mutation was shown to be responsible for the constitutive activation of molecular signaling pathways, leading to an uncontrolled cell proliferation in myeloproliferative neoplasms (MPN). This mutation is found in a large proportion of Philadelphia-Negative MPN patients with Polycythemia Vera (90%), Essential Thrombocythemia (35-70%) and, less frequently in patients with Primary Myelofibrosis (50%). The presence of the JAK2 V617F mutation is considered as a major criterion for diagnosis of MPN (WHO Guidelines 2008). The JAK2 allele burden decreases with successful therapy, disappear in some patients, and reappears during relapse. In patients with JAK2 V617F positive, treatment response is increasingly evaluated with minimal residual disease assays. Thus, Ana Gene Biotech has been developed and validated a real-time PCR assay for the quantitation of JAK2 V617F mutation to determine minimal residual disease (MRD) in patients with JAK2 V617F positive. This method detectes the mutation with results normalized to JAK2 normal gene as reference control, using the Taqman Probe technology. JAK2 V617V mutation and JAK2 normal are detected in the FAM channel and Internal control (ABL1 gene) is detected in the Yellow channel.

JAK2 MutID

- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Positive Control
- Water Nuclease free
- Quick Protocol
- mini CD


JAK2 MutID

I. Sample Requirement
DNA from Peripheral blood: 0.5 - 1mL
DNA from Bone from marrow: 0.5 - 1mL

II. Sensitivity
These reagents detect 10 copies of JAK2 V617F allele. Sensitivity of the assay was determined by serially
diluting the positive control of JAK2 V617F from 100 to 1 copy/PCR. Sensitivity of the assay was determined
as 10 copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be detected.
III. Module Specificity
Test result will be positive, only to JAK2 V617F mutation. The primers and probes were checked for possible
homologies to all published sequences (Genbank) by BLAST analysis to avoid any homology with other
organisms.
IV. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products. Thus, users
can get reproducible data even though they use different batch of product.
V. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer using
hematopoietic cell lines, blood positive and negative samples.
VI. Speed
Total reaction time is less than 2 hour.

JAK2 MutID

1. This Kit is intended for molecular biology applications and is not intended for the prevention or treatment of a disease.

2. All reagents may exclusively be used for in vitro diagnostics.

3. The product is to be used by personnel specially instructed and trained in for the in-vitro diagnostics procedures only.

4. It is important to pipet the indicated quantities, and mix well after each reagent addition. Check pipettes regularly.

5. Instructions must be followed correctly in order to obtain correct results.

6. This test has been validated for use with the reagents provided in the kit. The use of other Reagents or methods, or the
use of equipment not fulfilling the specifications, may render equivocal results.

7. Detection of translocation depends on the number of fusion transcripts present in the sample, and can be affected by
sample collection methods, patient-related factors (e.g. age, symptoms) or for infection stage and sample size.

8. Cross contamination between samples and exogenous DNA can only be avoided by following good laboratory practice.

9. Attention should be paid to expiration dates printed on the kit box. Do not use expired product.

JAK2 MutID

1. Tefferi A, et al. Classification and diagnosis of myeloproliferative neoplasms: the 2008 World Health Organization criteria and point-of-care diagnostic algorithms. 2008. Leukemia; 22:14-22.

2. Kr?ger N, et al. Monitoring of the JAK2-V617F mutation by highly sensitive quantitative real-time PCR after allogeneic stem cell transplantation in patients with myelofibrosis. 2007. Blood; 109:1316-1321.

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