Ana Gene Biotech
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Thrombophilia MutID kit

- Qualitative detection of most common thrombophilia mutations:
- FII (G20210A), FV (c.1691GA) known as FVL (factor V Leiden ), FV (A4070G) known as FVHR2, FXIII (G20210A)-(Val34Leu), MTHFR (C677T), MTHFR (A1298T), PAI-1 (5G/4G)

- Real-time PCR ARMS TaqMan Probe Technology,
- Highly Specific and Sensitive Detection Kit.

Catalog Number Description (12 samples)
- K2033: 2X version , 12 samples
- K2033L: Lyophilized version , 12 samples (containing Lyophilized qPCR Mixes)
Lyophilized Version Price :
$363
Old Price :
$0
Amount
    •  
    •  Jan 2017
    •  
    •  2017

Thrombophilia MutID kit

Thrombophilia or hypercoagulability is an inherited or acquired susceptibility to thrombosis (blood clots) due to abnormal coagulability of the clotting system. This panel provides analysis of a combination of acquired and inherited thrombophilia risk factors that have been shown to be associated with recurrent loss and other poor obstetric outcomes. Thrombophilia is defined as a predisposition for thrombosis which can arise from genetic factors, acquired changes in the clotting mechanism, or, more commonly, an interaction between genetic and acquired factors. Thrombophilia MutID Kit (Qualitative Mutation Analysis Real-time PCR Kit) is an in-vitro nucleic acid amplification test for the qualitative detection of following mutations in human clinical samples, simultaneously.
FII G20210A, FVL 1691GA, FXIII V34L, FVHR2 (FVH1299R), MTHFR C677T, MTHFR A1298T and PAI1 4G/5G
The kit contains all the reagents necessary for qualitative mutations analysis. The assay is based on Real time PCR with Taqman probe chemistry. The mutation and Internal Control are detected in the FAM and Yellow channel respectively. The Internal control is designed to efficiently control DNA extraction process and inhibition during the PCR amplification.


Thrombophilia MutID kit

- FII Normal qPCR Mix (2X)
- FII Mutant qPCR Mix (2X)
- FVL Normal qPCR Mix (2X)
- FVL Mutant qPCR Mix (2X)
- FVHR2 Normal qPCR Mix (2X)
- FVHR2 Mutant qPCR Mix (2X)
- FXIII Normal qPCR Mix (2X)
- FXIII Mutant qPCR Mix (2X)
- MTHFR677 Normal qPCR Mix (2X)
- MTHFR677 Mutant qPCR Mix (2X)
- MTHFR1298 Normal qPCR Mix (2X)
- MTHFR1298 Mutant qPCR Mix (2X)
- PAI1-5G qPCR Mix (2X)
- PAI1-4G qPCR Mix (2X)
- Positive Control Mix (PC)
- Dilution Buffer (only for lyophilized version)
- Water Nuclease free
- Quick Protocol
- mini CD


Thrombophilia MutID kit

I. Sensitivity
The Ct values of normal and mutant tubes should be before cycle 30 with appropriate quality and quantity of DNA (10-100 ng/reaction and optimum 20-80 ng/reaction). The Ct values above cycle 30 may be the result of nonspecific amplification or cross contamination (false positive) (for more details, refer to Appendix2- Ana Gene Real-time PCR Kit Troubleshooting). Furthermore, CT value difference between normal and mutant amplification plots should be ≤4. If it is 4 CT ≤7, test should be repeated. 7 CT value difference between normal and mutant amplification plots, considered to be normal. This result is due to nonspecific amplification or cross contamination (false amplification).
II. Module Specificity
Test result will be positive, only to related specific mutations. The primers and probes were checked for possible homologies to all published sequences (Genbank) by BLAST analysis to avoid any homology with other organisms.
III. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products. Thus, users can get reproducible data even though they use different batch of product.
IV. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer using hematopoietic cell lines, blood positive and negative samples.
V. Speed
Total reaction time is less than 2 hour.


Thrombophilia MutID kit

• This Kit is intended for molecular biology applications and is not intended for the prevention or treatment of a disease.
• All reagents may exclusively be used for in vitro diagnostics.
• The product is to be used by personnel specially instructed and trained in for the in-vitro diagnostics procedures only.
• Do not subject reagents to 5 freeze-thaw cycles. Store all reagents on ice throughout the experiment.
• It is important to pipette the indicated quantities, and mix well after each reagent addition. Check pipettes regularly.
• Instructions must be followed correctly in order to obtain correct results.
• This test has been validated for use with the reagents provided in the kit. The use of other Reagents or methods, or the use of equipment not fulfilling the specifications, may render equivocal results.
• Detection of mutation depends on the number of fusion transcripts present in the sample, and can be affected by sample collection methods, patient-related factors (e.g. age, symptoms) or for infection stage and sample size.
• Cross contamination between samples and exogenous DNA can only be avoided by following good laboratory practice.
• Attention should be paid to expiration dates printed on the kit box. Do not use expired product.


Thrombophilia MutID kit



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