Ana Gene Biotech
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Tamoxifen MutID kit

- Qualitative detection of FII (G20210A) and FV (c.1691GA) known as FVL (factor V Leiden )
- Real-time PCR ARMS TaqMan Probe Technology,
- Highly Specific and Sensitive Detection Kit.

Catalog Number Description (12 samples)
- K2034: 2X version , 12 samples
- K2034L: Lyophilized version , 12 samples (containing Lyophilized qPCR Mixes)
Lyophilized Version Price :
$195
Old Price :
$0
Amount
    •  
    •  Jan 2017
    •  
    •  2017

Tamoxifen MutID kit

The risk of developing a venous thromboembolism (VTE) is related to a number of acquired and inherited risk factors. These include use of exogenous estrogens or estrogen agonists (eg, hormone replacement therapy, oral contraceptives, tamoxifen), pregnancy, obesity, and inherited mutations in genes predisposing to these events.
Tamoxifen is an anti-estrogen used in the treatment and prevention of breast neoplasms particularly those with estrogen receptor positive breast cancer. However, the use of tamoxifen as a preventive agent may be limited by the increased risk of side effects. A new report from the Breast Cancer Prevention Project (BCPT) addresses whether FV Leiden (1691GA) and F II (G20210A) as two common thrombophilic mutations predisposed tamoxifen recipients to VTE. Therefore In the adjuvant setting, it may be prudent to test for FVL and FII (prothrombin) G2010A mutations before starting treatment with tamoxifen. The kit contains all the reagents necessary for qualitative mutations analysis. The assay is based on Real time PCR with Taqman probe chemistry. The mutation and Internal Control are detected in the FAM and Yellow channel respectively. The Internal control is designed to efficiently control DNA extraction process and inhibition during the PCR amplification.


Tamoxifen MutID kit

- FII Normal qPCR Mix (2X)
- FII Mutant qPCR Mix (2X)
- FVL Normal qPCR Mix (2X)
- FVL Mutant qPCR Mix (2X)
- Positive Control Mix (PC)
- Dilution Buffer (only for lyophilized version)
- Water Nuclease free
- Quick Protocol
- mini CD


Tamoxifen MutID kit

I. Sensitivity
These reagents detect 10 copies of FII and FVL mutations. Sensitivity of the assay was determined by serially diluting the positive control of DNA from 100 to 1 copy/PCR. Sensitivity of the assay was determined as 10 copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be detected.
II. Module Specificity
Test result will be positive, only to FII and FVL mutations. The primers and probes were checked for possible homologies to all published sequences (Genbank) by BLAST analysis to avoid any homology with other organisms.
III. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products. Thus, users can get reproducible data even though they use different batch of product.
IV. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer using hematopoietic cell lines, blood positive and negative samples.
V. Speed
Total reaction time is less than 2 hour.


Tamoxifen MutID kit

• This Kit is intended for molecular biology applications and is not intended for the prevention or treatment of a disease.
• All reagents may exclusively be used for in vitro diagnostics.
• The product is to be used by personnel specially instructed and trained in for the in-vitro diagnostics procedures only.
• Do not subject reagents to 3 freeze-thaw cycles. Store all reagents on ice throughout the experiment.
• It is important to pipette the indicated quantities, and mix well after each reagent addition. Check pipettes regularly.
• Instructions must be followed correctly in order to obtain correct results.
• This test has been validated for use with the reagents provided in the kit. The use of other Reagents or methods, or the use of equipment not fulfilling the specifications, may render equivocal results.
• Detection of mutation depends on the number of mutation in the sample, and can be affected by sample collection methods, patient-related factors (e.g. age, symptoms) and/or infection stage and sample size.
• Cross contamination between samples and exogenous DNA can only be avoided by following good laboratory practice.
• Attention should be paid to expiration dates printed on the kit box. Do not use expired product.


Tamoxifen MutID kit



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  • Product Code: 137
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