Ana Gene Biotech
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FXIII MutID Kit

- Detection of FXIII V34L mutation,
- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Real-time PCR ARMS TaqMan Probe Technology,
- Highly Specific and Sensitive Detection Kit.

Catalog Number Description (24 reactions)
- K2028: 2X version including 24 ready to use tubes contain 10 μl of qPCR mix 2X (two sets)
- K2028L: Lyophilized version including 24 ready to use lyophilized qPCR mix (two sets)
Lyophilized Version Price :
$100
Old Price :
$0
Amount
    •  
    •  Jan 2017
    •  
    •  2017

FXIII MutID Kit

Thrombophilia is a highly prevalent problem, genetic alterations of different blood components can directly
or indirectly influence the hemostatic balance and cause a prothrombotic state. More than 10 percent of
the population affected by one of the currently known genetic risk factors. Factor XIII (FXIII), also called
fibrin stabilizing factor, has a crucial role in the blood coagulation and fibrinolytic pathways. The FXIII
Val34Leu variant is a G-to-T transition in exon 2 of the gene encoding for FXIIIA, leading to a valine (Val)-
to-leucine (Leu) substitution at amino acid 34. This variant is common in White populations, with a
frequency of approximately 0.25-0.30. However, the frequency varies among ethnic groups, with the
lowest (0.01) in Japanese and the highest (0.40) in Pima Indians.
FXIII MutID Kit (Qualitative Mutation Analysis Real-time PCR Kit) is an in-vitro nucleic acid amplification
test for the qualitative detection of factor XIII V34L mutation in human clinical samples. The kit contains
all the reagents necessary for qualitative mutations analysis. The assay is based on Real time PCR with
Taqman probe chemistry. The mutation and Internal Control are detected in the FAM and Yellow channel
respectively. The Internal control is designed to efficiently control DNA extraction process and inhibition
during the PCR amplification.

FXIII MutID Kit

- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Positive Control
- Water Nuclease free
- Quick Protocol
- mini CD


FXIII MutID Kit

I. Sensitivity
These reagents detect 10 copies of Factor XIII. Sensitivity of the assay was determined by serially
diluting the positive control of DNA from 100 to 1 copy/PCR. Sensitivity of the assay was
determined as 10 copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be
detected.
II. Module Specificity
Test result will be positive, only to Factor XIII (V34L) mutation. The primers and probes were
checked for possible homologies to all published sequences (Genbank) by BLAST analysis to avoid
any homology with other organisms.

III. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products.
Thus, users can get reproducible data even though they use different batch of product.
IV. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer
using hematopoietic cell lines, blood positive and negative samples.
V. Speed
Total reaction time is less than 2 hour.

FXIII MutID Kit

This Kit is intended for molecular biology applications and is not intended for the prevention or
treatment of a disease.
All reagents may exclusively be used for in vitro diagnostics.
The product is to be used by personnel specially instructed and trained in for the in-vitro
diagnostics procedures only.
It is important to pipette the indicated quantities, and mix well after each reagent addition. Check
pipettes regularly.
Instructions must be followed correctly in order to obtain correct results.
This test has been validated for use with the reagents provided in the kit. The use of other Reagents
or methods, or the use of equipment not fulfilling the specifications, may render equivocal results.
Detection of mutation depends on the number of fusion transcripts present in the sample, and can be
affected by sample collection methods, patient-related factors (e.g. age, symptoms) or for infection
stage and sample size.
Cross contamination between samples and exogenous DNA can only be avoided by following good
laboratory practice.
Attention should be paid to expiration dates printed on the kit box. Do not use expired product.

FXIII MutID Kit

1. Breccia M, et al. Clinical and biological features of acute promyelocytic leukemia patients developing retinoic acid syndrome during induction treatment with all-trans retinoic acid and idarubicin. 2008. Haematologica; 93 (12): 1918-20.

2. Van Dongen JJM, et al. Standardized RT-PCR analysis of fusion gene transcripts from chromosome aberrations in acute leukemia for detection of minimal residual disease. 1999. Leukemia; 13: 1901-1928.

3. McCraw B, et al. Diagnosing disseminated intravascular coagulopathy in acute promyelocytic leukemia. 2008. Clinical Journal of Oncology Nursing


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