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FII MutID kit

- Detection of FII G20210A mutation,
- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Real-time PCR ARMS TaqMan Probe Technology,
- Highly Specific and Sensitive Detection Kit.

Catalog Number Description (24 reactions)
- K2029: 2X version including 24 ready to use tubes contain 10 μl of qPCR mix 2X (two sets)
- K2029L: Lyophilized version including 24 ready to use lyophilized qPCR mix (two sets)
Lyophilized Version Price :
Old Price :
    •  Jan 2017
    •  2017

FII MutID kit

When a patient has a tendency to form blood clots, the condition is called thrombophilia. It has been described as a major factor in the pathogenesis of some severe obstetric pathologies, e.g. deep venous thrombosis, pre-eclampsia and intrauterine growth retardation, as well as pregnancy failure.
Recent research suggests a possible correlation between inherited thrombophilia and recurrent fetal loss. Genetic markers for these clotting factors include factor V Leiden mutation and prothrombin G20210A mutation. Prothrombin is a protein in the blood that is needed for the blood to clot properly. It is also called factor II (two). Blood clots are made of platelets and the blood clotting protein ‘fibrin’. Prothrombin is a blood clotting protein that is needed to form fibrin. The prothrombin gene mutation causes your body to make too much prothrombin. This makes your blood more likely to form clots, which can be dangerous.
FII MutID Kit (Qualitative Mutation Analysis Real-time PCR Kit) is an in-vitro nucleic acid amplification test for the qualitative detection of factor II mutation in human clinical samples. The kit contains all the reagents necessary for qualitative mutations analysis. The assay is based on Real time PCR with Taqman probe chemistry. The mutation and Internal Control are detected in the FAM and Yellow channel respectively. The Internal control is designed to efficiently control DNA extraction process and inhibition during the PCR amplification.

FII MutID kit

- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Positive Control
- Water Nuclease free
- Quick Protocol
- mini CD

FII MutID kit

I. Sensitivity
These reagents detect 10 copies of Factor II. Sensitivity of the assay was determined by serially
diluting the positive control of DNA from 100 to 1 copy/PCR. Sensitivity of the assay was
determined as 10 copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be
II. Module Specificity
Test result will be positive, only to Factor II mutation. The primers and probes were checked for
possible homologies to all published sequences (Genbank) by BLAST analysis to avoid any
homology with other organisms.
III. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products.
Thus, users can get reproducible data even though they use different batch of product.
IV. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer
using hematopoietic cell lines, blood positive and negative samples.
V. Speed
Total reaction time is less than 2 hour.

FII MutID kit

This Kit is intended for molecular biology applications and is not intended for the prevention or
treatment of a disease.
All reagents may exclusively be used for in vitro diagnostics.
The product is to be used by personnel specially instructed and trained in for the in-vitro
diagnostics procedures only.
It is important to pipette the indicated quantities, and mix well after each reagent addition. Check
pipettes regularly.
Instructions must be followed correctly in order to obtain correct results.
This test has been validated for use with the reagents provided in the kit. The use of other Reagents
or methods, or the use of equipment not fulfilling the specifications, may render equivocal results.
Detection of mutation depends on the number of fusion transcripts present in the sample, and can be
affected by sample collection methods, patient-related factors (e.g. age, symptoms) or for infection
stage and sample size.
Cross contamination between samples and exogenous DNA can only be avoided by following good
laboratory practice.
Attention should be paid to expiration dates printed on the kit box. Do not use expired product.

FII MutID kit

1. Breccia M, et al. Clinical and biological features of acute promyelocytic leukemia patients developing retinoic acid syndrome during induction treatment with all-trans retinoic acid and idarubicin. 2008. Haematologica; 93 (12): 1918-20.

2. Van Dongen JJM, et al. Standardized RT-PCR analysis of fusion gene transcripts from chromosome aberrations in acute leukemia for detection of minimal residual disease. 1999. Leukemia; 13: 1901-1928.

3. McCraw B, et al. Diagnosing disseminated intravascular coagulopathy in acute promyelocytic leukemia. 2008. Clinical Journal of Oncology Nursing

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