Ana Gene Biotech
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PAI-1 MutID Kit

- Detection of PAI-1 4G/5G variation,
- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Real-time PCR ARMS TaqMan Probe Technology,
- Highly Specific and Sensitive Detection Kit.

Catalog Number Description (24 reactions)
- K2032: 2X version including 24 ready to use tubes contain 10 μl of qPCR mix 2X (two sets)
- K2032L: Lyophilized version including 24 ready to use lyophilized qPCR mix (two sets)
Lyophilized Version Price :
$100
Old Price :
$0
Amount
    •  
    •  Jan 2017
    •  
    •  2017

PAI-1 MutID Kit

Thrombophilia or hypercoagulability is an inherited or acquired susceptibility to thrombosis (blood clots)
due to abnormal coagulability of the clotting system. This panel provides analysis of a combination of
acquired and inherited thrombophilia risk factors that have been shown to be associated with recurrent
loss and other poor obstetric outcomes. Thrombophilia is defined as a predisposition for thrombosis which
can arise from genetic factors, acquired changes in the clotting mechanism, or, more commonly, an
interaction between genetic and acquired factors. About 40% of patients with thrombosis have inherited
the disease. Inherited thrombophilia is suspected in individuals with a history of venous thromboembolism
(VTE) manifest as deep vein thrombosis (DVT) or pulmonary embolism, especially in women with a
history of VTE during pregnancy or in association with oral contraceptive use, and in individuals with a
personal or family history of recurrent thrombosis. It is also associated with early and late recurrent
pregnancy loss as a result of uteroplacental microvascular thrombosis and hypoperfusion.
Homozygous abnormality or the combination of two or multiple hetrozygous abnormal factors can lead to
clinical apparent thrombotic disorders at an early age.

Thrombophilia MutID Kit (Qualitative Mutation Analysis Real-time PCR Kit) is an in-vitro nucleic acid
amplification test for the qualitative detection of below mutations in human clinical samples,
simultaneously. The kit contains all the reagents necessary for qualitative mutations analysis. The assay is
based on Real time PCR with Taqman probe chemistry. The mutation and Internal Control are detected in
the FAM and Yellow channel respectively. The Internal control is designed to efficiently control DNA
extraction process and inhibition during the PCR amplification.

PAI-1 MutID Kit

- 24 Ready to use Lyophilized tubes (lyophilized Version kit)
- 24 Ready to use 2X tubes (2X Version kit)
- Positive Control
- Water Nuclease free
- Quick Protocol
- mini CD


PAI-1 MutID Kit

I. Sensitivity
These reagents detect 10 copies of PAI1-4G mutation. Sensitivity of the assay was determined by serially diluting the positive control of DNA from 100 to 1 copy/PCR. Sensitivity of the assay was determined as 10 copies/PCR. This means that there is 95% probability that 10 Copies/PCR will be detected.
II. Module Specificity
Test result will be positive, only to PAI1-4G. The primers and probes were checked for possible homologies to all published sequences (Genbank) by BLAST analysis to avoid any homology with other organisms.
III. Reproducibility
Systematic kit production and strict quality control system eliminate batch variation of products. Thus, users can get reproducible data even though they use different batch of product.
IV. Quality Control
Functionality tested in PCR for specificity, sensitivity and reproducibility for specific probe/primer using hematopoietic cell lines, blood positive and negative samples.
V. Speed
Total reaction time is less than 2 hour.


PAI-1 MutID Kit

• This Kit is intended for molecular biology applications and is not intended for the prevention or treatment of a disease.
• All reagents may exclusively be used for in vitro diagnostics.
• The product is to be used by personnel specially instructed and trained in for the in-vitro diagnostics procedures only.
• Do not subject reagents to > 3 freeze-thaw cycles. Store all reagents on ice throughout the experiment.
• It is important to pipette the indicated quantities, and mix well after each reagent addition. Check pipettes regularly.
• Instructions must be followed correctly in order to obtain correct results.
• This test has been validated for use with the reagents provided in the kit. The use of other Reagents or methods, or the use of equipment not fulfilling the specifications, may render equivocal results.
• Detection of mutation depends on the number of mutation in the sample, and can be affected by sample collection methods, patient-related factors (e.g. age, symptoms) and/or infection stage and sample size.
• Cross contamination between samples and exogenous DNA can only be avoided by following good laboratory practice.
• Attention should be paid to expiration dates printed on the kit box. Do not use expired product.


PAI-1 MutID Kit



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